Table1. Toxicity (LC50 values) of ASBME and LIBME against freshwater snail Lymnaea acuminata at different time
intervals in pond.
|
Exposure periods
|
Effective dose (mg/L)
|
Limits (mg/L)
|
Slope value
|
‘t’ ratio
|
Hetero-geneity
|
|
LCL
|
UCL
|
|
Alstonia scholaris methanol extract
|
|
24h
|
LC50 = 55.19
|
47.67
|
70.06
|
2.19±0.36
|
6.01
|
0.50
|
|
48h
|
LC50 = 40.29
|
36.40
|
45.22
|
2.65±0.35
|
7.38
|
0.55
|
|
72h
|
LC50 = 31.48
|
26.64
|
36.00
|
1.96±0.34
|
5.71
|
0.14
|
|
96h
|
LC50 = 23.20
|
9.73
|
30.38
|
2.45±0.36
|
6.82
|
0.10
|
|
Lantana indica methanol extract
|
|
24h
|
LC50 = 41.28
|
34.18
|
57.75
|
1.71±0.32
|
5.33
|
0.46
|
|
48h
|
LC50 = 30.35
|
25.88
|
37.73
|
1.66±0.31
|
5.35
|
0.11
|
|
72h
|
LC50 = 21.38
|
18.15
|
24.48
|
1.96±0.31
|
6.32
|
0.38
|
|
96h
|
LC50 = 14.73
|
6.92
|
19.43
|
2.52±0.33
|
7.61
|
0.10
|
·
Hundred snails were exposed to four
different concentrations of the extract.
·
Concentrations given are the final
concentration (w/v) in the de-chlorinated water.
·
Mortality was determined at every 24h.
·
Regression coefficient showed that
there was significant (P<0.05) negative
·
Regression between exposure time and
different LC values.
·
LCL-Lower confidence limit; UCL-Upper
confidence limit.
·
There was no mortality in control
group.
Table2. Toxicity (LC50 values) of binary mixture of LIBME +ASBME against freshwater snail Lymnaea acuminata at different time
intervals in pond.
|
Exposure periods
|
Effective dose (mg/L)
|
Limits (mg/L)
|
Slope value
|
‘t’ ratio
|
Hetero-geneity
|
|
LCL
|
UCL
|
|
Lantana indica+Alstonia scholaris Mthanol
(1:5 ratio)
|
|
24h
|
LC50
= 28.53
|
27.56
|
30.24
|
9.31±1.63
|
5.70
|
0.19
|
|
48h
|
LC50
= 27.02
|
26.28
|
28.01
|
9.76±1.59
|
6.10
|
0.81
|
|
72h
|
LC50
= 24.86
|
23.79
|
25.66
|
8.33±1.57
|
5.29
|
0.02
|
|
96h
|
LC50
= 23.65
|
22.87
|
24.23
|
13.73±1.74
|
7.88
|
0.22
|
|
Lantana indica+Alstonia scholaris Methanol (1:1 ratio)
|
|
24h
|
LC50
= 22.76
|
21.74
|
24.62
|
7.25±1.26
|
5.73
|
0.39
|
|
48h
|
LC50
= 20.59
|
19.77
|
21.65
|
6.50±1.21
|
5.37
|
0.89
|
|
72h
|
LC50
= 18.87
|
17.92
|
19.61
|
6.87±1.21
|
5.67
|
0.23
|
|
96h
|
LC50
= 17.78
|
16.96
|
18.38
|
9.65±1.29
|
7.43
|
0.84
|
Other details are given in
Table 1.