Argonauta 1-6 2010
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Table3: Changes in total free amino acids, total protein, glycogen level, and nucleic acid level (DNA and RNA) in nervous and hepatopancreas tissues of freshwater snail Lymnaea acuminata after exposure to sub-lethal doses (40% and 80% of LC50 of 24h) of methanolic extract of stem bark of Lantana indica after 96h exposure periods in pond.
|
Parameter |
Tissue |
Control |
40% of LC50 |
80% of LC50 |
7 days of the withdrawal |
|
Amino acid |
Nervous |
17.0± 0.01 (100) |
20.0± 0.01* (118) |
23.0±0.01* (135) |
18.6±0.03** (109) |
|
Hepatopancreas |
18.2± 0.01 (100) |
22.6±0.01 * (124) |
25.1±0.01* (138) |
19.5±0.02** (107) |
|
|
Protein |
Nervous |
21.0 ± 0.01 (100) |
16.4±0.01* (78) |
12.4±0.01* (59) |
19.7±0.02** (94) |
|
Hepatopancreas |
25.0± 0.01 (100) |
19.0±0.01* (76) |
14.0±0.01* (56) |
23.9±0.01** (96) |
|
|
Glycogen |
Nervous |
1.4± 0.01 (100) |
1.0±0.01* (71) |
0.7±0.01* (50) |
1.3±0.01** (93) |
|
Hepatopancreas |
1.1± 0.01 (100) |
0.77±0.01* (64) |
0.52±0.01* (47) |
1.0±0.01** (91) |
|
|
DNA |
Nervous |
12.4± 0.01 (100) |
10.3±0.01* (83) |
8.2±0.01* (66) |
11.5±0.01** (93) |
|
Hepatopancreas |
13.0± 0.01 (100) |
9.9±0.01* (76) |
8.5±0.01* (65) |
12.4±0.01** (95) |
|
|
RNA |
Nervous |
19.3± 0.01 (100) |
14.4±0.01* (75) |
12.1±0.01* (63) |
18.6±0.02** (96) |
|
Hepatopancreas |
17.8± 0.01 (100) |
13.0±0.01* (73) |
10.7±0.01* (60) |
16.9±0.01** (95) |
|
|
Protease
|
Nervous |
0.32±0.02 (100) |
0.41±0.01* (128) |
0.45±0.02* (141) |
0.34±0.01** (106) |
|
Hepatopancreas |
0.46±0.01 (100) |
0.55±0.01* (120) |
0.63±0.03* (137) |
0.50±0.01** (109) |
· All these values are ± SE of six replicates.
· Values in parenthesis are percent change with control taken as 100%.
· Data were analysed through student’s‘t’ test.
· *, shows significant (P<0.05) changes, when treated groups were compared with controls.
· **, shows significant (P<0.05). Student ‘t’ test was applied between 80% of 24h LC50 and withdrawal.
· Levels of total free amino acids, total protein, nucleic acid (DNA and RNA) are expressed in µg/mg and glycogen level are expressed in mg/g. and activity of protease in micromoles of tyrosine equivalents/mg protein /h
Fig. 1. Bar diagram showing the percent level of
total free amino acid (µg/mg), total protein (µg/mg), glycogen (mg/g), DNA and
RNA (µg/mg), protease level (µmol tyrosine/mg protein/h) in nervous and
hepatopancreas (HP) tissue of the freshwater snail Lymnaea acuminata after 96h
exposure to 40% and 80% of LC50 (24h) of methanolic extract
of stem bark of Alstonia scholaris and 7 days of the withdrawal.
Fig.2. Bar diagram showing the percent level of
total free amino acid (µg/mg), total protein (µg/mg), glycogen (mg/g), DNA and
RNA (µg/mg), protease level (µmol tyrosine/mg protein/h) in nervous and
hepatopancreas (HP) tissue of the freshwater snail Lymnaea acuminata after 96h
exposure to 40% and 80% of LC50 (24h) of binary combination of methanolic
extract of Lantana indica+Alstonia
scholaris stem bark in 1:1 ratio, and 7 days of the withdrawal.